hii all
i'm asking why multiple codons code for the same amino acid but different protein activity ?
please attach papers or reviews
thank you all
zaynab
You can take a look at Wobble base pair
A wobble base pair is a pairing between two nucleotides in RNA molecules that does not follow Watson-Crick base pair rules.
The wobble position of a codon refers to the 3rd nucleotide in a codon. This nucleotide has two major characteristics:
For further reading: https://en.wikipedia.org/wiki/Wobble_base_pair
Are you referring to "codon bias" where there are differences in the frequencies of synonymous codons?
Here's a very nice publication that addresses that codon bias and how that affects protein expression:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4794256/
Thanks for reply
if silence mutation occured but both( before and after mutation) the codon gives the same protein but this protein has different activity 🤔
The change in codon may affect the level of expression of the enzyme, which would appear as a difference in the activity of the protein in a crude extract if the different amount of enzyme is not taken into account. However, if the proteins are purified to equal purity, they should have the same activity. Differences in activity between two preparations of the same protein can occur because of differences in the method used for purification or differences in handling of the protein during purification or storage.
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