Mice database deposition

More Sergey Kolomeychuk's questions See All

NGS vs microarray. Could you recommend better choice?

Dear colleagues, I have tissue samples to analyze gene expression. Could youre commend me better choice, next generation sequensing or microarray?

10 November 2014 4,336 3 View

How long are lyophilized primers viable after being stored at -20C?

I have two tubes with lyophilized primers. My boss forgot about them and found after 3 years at -20C. Is it worth checking them or better order new ones?

01 February 2014 6,234 12 View

Is it possible to perform promoter methylation and immunohistochemistry studies on formalin-fixed paraffin-embedded tissue samples?

Dear colleagues, I have received many FFPE samples for analysis. Half of them represent normal tissue, the other half - cancerous. I have already done gene expression. Is it possible to perform...

09 October 2013 1,418 0 View

How to perform an analysis of DNA methylation?

What is preferable for the analysis of DNA methylation: skin samples or oral mucosa?

09 October 2013 7,747 2 View

Can someone help with SNP validation by Haploview?

I've got data from a cross-sectional study on heart disease. I've got 4 SNPs on one gene and need to calculate the LD, D, and r2. I've installed Haploview but don t know how to upload my data and...

04 May 2013 9,272 4 View

Mouse tail length

Dear colleagues, please help me. We are performing experiment for modeling obesity in mice. Is that possible to measure mouse tail and correlate it to total length of the animal?

02 March 2013 4,223 2 View

The best antibody for CLOCK, PER1 and CRY proteins

Please help me to find out the best source for human antibodies to detect CLOCK, PER1 and CRY proteins in tissue and blood samples.

31 December 2012 6,396 0 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What should Berlin do as a city to become as impactful as London and Paris in World Football?

Please go through my Abstract. I can also share a proposed Thesis Outline.

04 August 2024 2,077 0 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

Why can't academics earn the money they deserve?

Only Journals make money from the articles we have worked on for years. Academics do not earn money from their refereeing. Then shouldn't the solution be a system in which academics can earn...

01 August 2024 6,469 6 View

Posthoc test lettering in JAMOVI?

Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...

31 July 2024 3,333 4 View

What are the roles of innovation in achieving the Sustainable Development Goals (SDG)?

31 July 2024 3,533 2 View