NGS vs microarray. Could you recommend better choice?

Dear colleagues, I have tissue samples to analyze gene expression. Could youre commend me better choice, next generation sequensing or microarray?

10 November 2014 4,215 3 View

How long are lyophilized primers viable after being stored at -20C?

I have two tubes with lyophilized primers. My boss forgot about them and found after 3 years at -20C. Is it worth checking them or better order new ones?

01 February 2014 6,125 12 View

Is it possible to perform promoter methylation and immunohistochemistry studies on formalin-fixed paraffin-embedded tissue samples?

Dear colleagues, I have received many FFPE samples for analysis. Half of them represent normal tissue, the other half - cancerous. I have already done gene expression. Is it possible to perform...

09 October 2013 1,313 0 View

How to perform an analysis of DNA methylation?

What is preferable for the analysis of DNA methylation: skin samples or oral mucosa?

09 October 2013 7,641 2 View

Can someone help with SNP validation by Haploview?

I've got data from a cross-sectional study on heart disease. I've got 4 SNPs on one gene and need to calculate the LD, D, and r2. I've installed Haploview but don t know how to upload my data and...

04 May 2013 9,168 4 View

Mouse tail length

Dear colleagues, please help me. We are performing experiment for modeling obesity in mice. Is that possible to measure mouse tail and correlate it to total length of the animal?

02 March 2013 4,096 2 View

The best antibody for CLOCK, PER1 and CRY proteins

Please help me to find out the best source for human antibodies to detect CLOCK, PER1 and CRY proteins in tissue and blood samples.

31 December 2012 6,289 0 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

No title

01 March 2021 3,905 5 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View