Please help me to find out the best source for human antibodies to detect CLOCK, PER1 and CRY proteins in tissue and blood samples.
Dear colleagues, I have tissue samples to analyze gene expression. Could youre commend me better choice, next generation sequensing or microarray?
10 November 2014 4,215 3 View
I have two tubes with lyophilized primers. My boss forgot about them and found after 3 years at -20C. Is it worth checking them or better order new ones?
01 February 2014 6,125 12 View
Dear colleagues, I have received many FFPE samples for analysis. Half of them represent normal tissue, the other half - cancerous. I have already done gene expression. Is it possible to perform...
09 October 2013 1,313 0 View
What is preferable for the analysis of DNA methylation: skin samples or oral mucosa?
09 October 2013 7,641 2 View
Together with our collaborators we receive data about sensitivity of several mouse strains to TNF. Now we want to deposit it in databases. Could you recommend a database and workflow of deposition?
05 June 2013 5,009 2 View
I've got data from a cross-sectional study on heart disease. I've got 4 SNPs on one gene and need to calculate the LD, D, and r2. I've installed Haploview but don t know how to upload my data and...
04 May 2013 9,168 4 View
Dear colleagues, please help me. We are performing experiment for modeling obesity in mice. Is that possible to measure mouse tail and correlate it to total length of the animal?
02 March 2013 4,096 2 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...
03 March 2021 8,920 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...
02 March 2021 10,003 2 View
I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.
02 March 2021 4,359 3 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View