Please help me to find out the best source for human antibodies to detect CLOCK, PER1 and CRY proteins in tissue and blood samples.
Dear colleagues, I have tissue samples to analyze gene expression. Could youre commend me better choice, next generation sequensing or microarray?
10 November 2014 4,336 3 View
I have two tubes with lyophilized primers. My boss forgot about them and found after 3 years at -20C. Is it worth checking them or better order new ones?
01 February 2014 6,234 12 View
Dear colleagues, I have received many FFPE samples for analysis. Half of them represent normal tissue, the other half - cancerous. I have already done gene expression. Is it possible to perform...
09 October 2013 1,418 0 View
What is preferable for the analysis of DNA methylation: skin samples or oral mucosa?
09 October 2013 7,747 2 View
Together with our collaborators we receive data about sensitivity of several mouse strains to TNF. Now we want to deposit it in databases. Could you recommend a database and workflow of deposition?
05 June 2013 5,122 2 View
I've got data from a cross-sectional study on heart disease. I've got 4 SNPs on one gene and need to calculate the LD, D, and r2. I've installed Haploview but don t know how to upload my data and...
04 May 2013 9,272 4 View
Dear colleagues, please help me. We are performing experiment for modeling obesity in mice. Is that possible to measure mouse tail and correlate it to total length of the animal?
02 March 2013 4,223 2 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View