I have two tubes with lyophilized primers. My boss forgot about them and found after 3 years at -20C. Is it worth checking them or better order new ones?
It is worth checking then. liophilized they are very stable (more than disolved) and i would even check 3 years old disolved oligos.
Best
GAD
It is worth checking then. liophilized they are very stable (more than disolved) and i would even check 3 years old disolved oligos.
Best
GAD
if current of-20C has not fluctuated, go for single PCR reaction, by different Primer conc... if result comes good use it.
They would even work, if they were dissolved stock concentrations kept at -20°C. I used primer stocks which where much older than this without problems.
They should be OK. As said Guido, lyophilization make primers very stable for a long time. But test them first, as usual:-)
Good luck,
Lenka
I also agree with Guido Drexler. Because they are liophilized and not diluted.
I agree with Christian above; they should work even if they were stored dissolved in water. I routinely use 2 and 3 year old primer stocks stored in the freezer of a domestic frost-free refrigerator without problems, and have occasionally used much older stocks (nothing fancy: conventional PCR, QuikChange) successfully.
Shelf life of printers on the bench is 6+ months. DNA is extremely stable, Even if done of the oligionucleotides have degraded there should still be plenty remaining for PCR top take place.
I agree with the above collegues. I also have tried disolved primers in water after 3-4 years and they worked fine. A single PCR will solve the mystery...
Don't worry dear. It will work. Just cheer and do your experiments with it. I m 100% sure. I have also experienced the same as Panagoitis has. Make sure there was not a single hint of moisture inside the lyophilised primer vials before diluting it.
- Badges
- Science method