Methanolic extract of Albatrellus flettii is only soluble in methanol.But it has shown good cytotoxic activity against different cancer cells lines.
After running my extract through sephadex LH-20 column. i just found out that my column fractions are only soluble in 100 percent methanol.
how i can proceed for MTT with these fraction.
Need your suggestions.
Dear Almas Yaqoob,
Please have a look on these articles
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3609226/
http://www.scielo.br/pdf/bjps/v46n2/v46n2a20.pdf
But i think it is not allowed to used methanol 100% as the solvent in MTT, it must has effect on the cancer cell. then you can not know if the results your will get is due to your compound or due to the effect of the methanol.
but if you know that the active principle in your extract are solid in nature you can get rid of methanol by lyophilization and use the produced powder for the MTT assay. of course it will be soluble in DMSO and i hope no precipitation will happen on dilution with water
In addition to the above comments, if there is no other way out you should use respective methanol concentrations as control also. It could be like..
1. Cells in growth medium
2. Cells in methanol
3. Cells treated with drug
Then you can compare the difference in group 2 and 3 to find the effect of methanol. BTW 100% methanol is not recommended..
Dear Almas Yaqoob,
i used DMSO to dissolve polar methanol extracts before.
http://www.mdpi.com/1660-3397/15/5/133/htm
using 100% methanol is not recommended unless you want to dissolve the extract in methanol and then dilute in media before adding to the cell line (I always use a final concentration of
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