Dear Asim Ali Yaqoob

the optimal period to record the polarization curve should be at the stable final performance of continuous flow mode microbial fuel cell, or at the best performance cycle of batch mode.

Dear Asim,

You can measure different overpotentials by obtaining the polarization curve. These overpotentials include activation, ohmic, and concentration losses. The occurrence of the overshoot phenomenon can also be investigated by studying the polarization curve. To draw this curve, all you need to do is obtaining a stable potential for each external resistance. Reaching the stable potential is affected by various factors. These may include the strain's type, bio-electrochemical system assembly, type of operation, etc.

You might also find these papers interesting:

Article Microbial Fuel Cells: Methodology and Technology †

Article Single chamber microbial fuel cell with spiral anode for dai...

Article Characterization of a Microfluidic Microbial Fuel Cell as a ...

Best regards,

Hossein