May i know is there necessary to verify the presence of nerve impulse for PD in-vitro model (undifferentiated SH-SY5Y cell line)?

26 December 2017 1 1K Report

May I know is there necessary to verify the presence of nerve impulse for PD in-vitro model (undifferentiated SH-SY5Y cell line)?

Cássio Prinholato da Silva

PaTCH CLAMP can be useful

Badges
Science topic

More Peiling Yeo's questions See All

How to extract RNA from neurons?

Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...

23 July 2024 6,783 1 View

Is there any good protocols for dissect VG nerve?

Hi, I'm a student who studies neuroimmunology. I want to analyze mouse vagus nerve with RT-qPCR like this thesis but I don't know how to dissect the vagus nerve I found some protocol videos for...

10 June 2024 2,123 1 View

How do you keep tissue cold in tissuelyser ll when you extract RNA from animal tissue?

Hi, I'm a new master's student who has been studying mouse IPF model, and I read that Trizol with tissue needs to be cold. But I can't keep the tissue cold while I homogenizing it with...

30 May 2024 8,603 3 View

Could you advice me about rapid sample freezing with LN2, Isopentane?

Hi, I am a fresh master's student who just started to study Idiopathic Pulmonary fibrosis(IPF), and I am here to ask about how to freeze lungs with LN2 when I harvested lungs from mice because...

03 April 2024 2,213 1 View

Can you recommend me a good antibody for IL-6 Western blot or RT-PCR?

Hi, I am an MS student who is studying idiopathic Pulmonary fibrosis(IPF) My P.I asked to analyze cytokine IL-6 or TNF-a through Western blot despite Many researchers using ELISA for it because of...

26 March 2024 5,593 3 View

Is there any method to check Vagus nerve's activity with staining or markers?

I have been studying about Vagus nerve and I want to check the activity of the Vagus nerve in my research. Could you advice me about it?

17 March 2024 8,145 1 View

Sequence alignment software for Mac?

Hello all. I have recently updated my Mac OS and came to realise that some of the softwares that I have been using for my sequence alignment and editing are no longer compatible with the new...

19 December 2022 186 3 View

How do i culture medulloblastoma cell lines?

I have to run some experiments on D283 and D341 medulloblastoma cell lines, but have no experience culturing these cell lines. I have only ever handled 293Ts (adherent) and various AML/CML cell...

25 May 2022 1,960 0 View

Canonical Correspondence Analysis - How much data sets?

Hi there, I am planning to correlate microbial community with environmental variables using CCA. Is there any minimum for data replication? How many times should I go for sampling to per site (...

10 April 2022 5,233 3 View

Will protease and phosphatase inhibitors still work after it's expiry date?

Hi all, my lab currently uses thermofisher's Pierce protease and phosphatase inhibitor mini tablet. I noticed that the expiry date was back in May 2020. I keep the tablets at 4°C, and dissolve 1...

28 October 2021 1,483 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Project/Experimental assistance ？?

Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...

05 August 2024 5,829 1 View