Hi there,
I am planning to correlate microbial community with environmental variables using CCA. Is there any minimum for data replication? How many times should I go for sampling to per site ( assuming I have 5 sites ) to allow CCA? or would one time sampling be sufficient?
Thank you
First, you could consider how much time and money you have available to take the samples and analyse them. This may provide an answer. Another consideration is that you may be more interested in biological variation and less in technical variation, so actual replicates of sites may be of less importance but increasing the number of sites could be more important. Especially when establishing the relationship between species abundance and environmental variables.
J. A. Hageman does this mean that to use CCA, there is no minimum replication needed? in that case, i agree with adding more site.
Various rules of thumb would suggest 5-20 observations per variable. Thus, I think five would be the minimum. The best answer may be to include as many as you have the finances to collect.
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