Hi, I am an MS student who is studying idiopathic Pulmonary fibrosis(IPF)
My P.I asked to analyze cytokine IL-6 or TNF-a through Western blot despite Many researchers using ELISA for it because of tight budget, can you recommend good IL-6 antibody products for W.B? (It would be better if I could use it at ELISA)
Also, mRNA expression from RT-PCR method is fine too.
Thank you for reading my question.
Dear Dr. Eunseo Yeo
I would recommend CST for IL-6 antibody.
https://www.cellsignal.com/products/primary-antibodies/il-6-d5w4v-xp-rabbit-mab/12912
https://www.cellsignal.com/products/primary-antibodies/il-6-d3k2n-rabbit-mab/12153
I hope my previous articles will be helpful for your primer order.
Kind regards
AB Bayazid
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Hi Eunseo Yeo
* To assess the cytokines levels such as IL-6 quantification, ELISA is the preferred method due to its simplicity, speed, high sensitivity, and quantitative nature while immunoblotting could be complex and requires higher concentration of loading volume to measure the cytokine expression.
If you need antibodies for ELISA and Immunoblot both. These could work.
1. https://www.rndsystems.com/products/rat-il-6-antibody_af506#:~:text=Rat%20IL%2D6%20Antibody%20Summary&text=Detects%20rat%20and%20mouse%20IL,human%20IL%2D6%20is%20observed.
2. https://www.abcam.com/products/primary-antibodies/il-6-antibody-ab6672.html
Hope it helps,
Thanks,
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