Hi, I'm a student who studies neuroimmunology.
I want to analyze mouse vagus nerve with RT-qPCR like this thesis but I don't know how to dissect the vagus nerve I found some protocol videos for DRG neurons from a mouse but there are not many protocols for dissecting VG, and even there are no protocol video for VG nerve dissection in Youtube. Could you recommend any good protocol for VG dissection?
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RNA isolation from mouse DRG and JG/NG (VG) and qRT-PCR
Mouse DRG or nodose ganglia (NG)/jugular ganglia (JG) (combined VG) were harvested and homogenized with a bead homogenizer (BioSpec) in lysis buffer RA1 (Macherey-Nagel). Total RNA was extracted with the NucleoSpin RNA isolation kit following manufacturer’s protocol. Equal amounts of cDNA were synthesized from total RNA extracts using the iScript cDNA Synthesis kit. Gene expression levels were determined using gene-specific primers (key resources table) and 2X qPCR Universal Green MasterMix (Lamda Biotech) or Power SYBR™ Green PCR Master Mix (Thermo Fisher Scientific). Reactions were cycled using a StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific) or QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) using the manufacturer’s protocol. Gene expression was normalized to Gapdh, and relative expression was calculated using the ΔΔCt method.
DOI: https://doi.org/10.1016/j.cell.2023.11.027
It is accessible easily in the neck region. you may need a dissection lens since its pretty small in your model animals