Hello,
I used DNS method to determine xylanase activity of my aspergillus, i used this equation to calculate the activity,
xylanase activity = (mg of liberated xylose/ 0.15013 mg/Umol) / (V x T)
where V is the volume of my supernatant = 0.1ml
and T is the time of the reactions (5 mins at 50°C)
thank you
The calculation seems right, and the value is around the expected for some Aspergillus, as observed by Cunha et al.
Article Optimization of Xylanase Production from Aspergillus foetidu...
You have not told us what kind of activity it is you are reporting. Is it total activity or specific activity? However, given the units (U/mL), I believe it is total xylanase activity. Unlike Lorenzo, I think you have every cause to worry. Please reproduce the experiment. The producing mold could simply be special and you do not really have to worry. Your enzyme activity is very high.
Maurice Ekpenyong, yes it is the total xylanase activity.
I repeat the test more than once, the DO is the same,
I think the problem is in the equation...
Lorenzo Briganti thank you for your answer
The maximum value of xylanase activity reported by Cunha is 13.98 U/mL.
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