Hello dear colleagues, what kind of research I can conduct on Aspergillus fumigatus isolates apart from genotyping?

06 February 2024 0 3K Report

More Bahroz Mahmood's questions See All

How can I define Planetary motion in ABAQUS CAE, how define motion of three rollers around a central axis and passive rotation about their own axis?

16 July 2024 4,145 1 View

How can I synthesize beads of silica?

Hi to all of you! My goal is to create synthetic silica beads. These beads ought to have a specified size. Could someone please advise on how to proceed or give me the articles I need to finish my...

02 July 2024 8,170 1 View

How to find the incident spectra in Lumerical FDTD?

Hello, I am having a difficult time finding the incident plane wave wavelength dependent spectra of the lights source to find the EQE. Please advise.

26 June 2024 7,274 1 View

What is the procedure of Molecular Diagnosis of Friedreich Ataxia?

Hello I am seeking detailed protocols for the molecular diagnosis of Friedreich Ataxia. Best wishes Saqib

13 May 2024 1,448 2 View

How to induce hypothyroidism in Wistar Rats?

which is the most successful and guaranteed method, cause I Tried sodium fluoride 20mg/kg and it didn't work

08 April 2024 8,414 0 View

What are the applications of long duration (2-day, 3-day) Intensity duration frequency (IDF) curves?

IDF curves usually developed using the sub-daily rainfall data. If I use daily data and construct 2,3-day IDF curve, then what are the practical applications of such long duration curves? Thank you.

28 March 2024 918 0 View

Request assistance in assessing the environmental impact of municipal solid waste management using the LCA method and using an application program?

I need an expert to help me in assessing the environmental impact of Municipal Solid Waste Management using one of the following programs: SimaPro. ,GaBi. ,OpenLCA. ,Wrate or any suitable software...

20 February 2024 7,755 2 View

How to prevent recombinant protein from GSH abduct incorporation?

Hi, experts. I'm trying to expressed recombinant transmembrane protein incorporated with nucleophilic non-canonical amino acids(ncAAs) by genetic code expansion (GCE) in E.coli. I replace tyrosine...

03 February 2024 2,403 0 View

What are the new methods used to clarify the agricultural plan?

With climate change, high temperatures, and scarcity of rain in Iraq, we find it difficult to determine future agricultural plans based on the abundance of water and temperatures and providing...

25 January 2024 7,412 3 View

How to inhibit Glutathion(GSH) protein modification in E.coli?

Hi, i am working on unnatural Amino Acid (UAAs) incorporated Insulin receptor substrate (IRS-1) expression and purification in BL21(DE3) strain. While i expressed my protein with UAA Glutathion...

23 January 2024 1,397 4 View

Why my gel electrophoresis have shadow bands? Please see the attached picture for the gel electrophoresis ?

Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...

19 July 2024 148 6 View

Is still the cryptococcus sp. considered as the top most critical priority pathogen?

In the year 2022 the WHO released a fungal priority pathogen list in which Cryptococcus neoformans being placed at the top most of critical priority pathogen. Is it still a threat to human...

10 June 2024 4,055 3 View

How can i select positive homozygous and heterozygous controls for SNP genotyping?

Two genes are PNPLA3 and TM6SF2

01 June 2024 8,047 1 View

How to perform DNA extraction for Long read sequencing using QIAgen genomic buffer set kit from Aspergillus isolates?

Greetings everyone, I am currently tasked with conducting DNA extraction from Aspergillus isolates, utilizing the QIAgen genomic buffer set kit. Regrettably, not all the components outlined in the...

03 May 2024 4,985 0 View

BLAST sequence in plus/minus and plus/plus in reverse sequence when CRISPR is performed. Are they mutants?

We are performing CRISPR on a gene from a strain of Aspergillus niger and then sequencing the results by Sanger. When we compare the gene sequence with the resulting sequence we observe in the...

25 April 2024 6,159 0 View

How do I choose a suitable connection network for sPCA?

I am currently running a sPCA on GBS data from a number of plant populations following the tutorial provided by Thibaut Jombart. I understand one important decision to make during the analysis is...

04 April 2024 7,959 0 View

Why the fermentation process of Aspergillus niger does not produce citric acid?

Hello, everyone. When I use CICC 40553 Aspergillus niger (strain introduction can produce citric acid) to ferment corn meal and glucose, the fermentation condition is 30 ℃, 200 rpm, and the...

29 March 2024 9,299 4 View

Re perform PCR reaction on the same first one for genotyping?

I performed genotyping analysis. Some samples give a result of one SNP but didnt give a result for another SNP. Note, these SNPs are performed at the same plate (same condition). Depending on the...

26 March 2024 7,013 0 View

Genotyping plants for overexpression insertion?

I have plants (L. japonicus) that have been transformed with an overexpression plasmid. How can I know that these plants are homozygous for the insertion?

24 March 2024 8,533 2 View

What is the normal disc size of aspergillus and trichoderma specie?

I have to inoculate 6 discs of Trichoderma and aspergillus species in Potato dextrose broth in order to make the cell pellets of them .How do I measure or know the size of fungi discs .

13 March 2024 991 3 View