Freeze dring itself is a physical process to remove water molecules, so will not. However, care should be taken to ensure that the sample is completely dry. Samples which contain lots of sugar can be very diffciult to freeze properly, therefore the sample will not dry well. You may see a gum, rather than nice powder. A gum can be hard to redissolve before analysis.

General guide to freeze drying can be found here:

https://scientificproducts.com/basic-principles-of-freeze-drying/

Thank you very much Rob

Despite the success of freeze drying in eliminating water molecules through sublimation, ensuring thorough dryness is crucial. However, samples with high sugar content pose a challenge as they do not freeze well, resulting in a sticky residue rather than a finely powdered substance. The difficulty lies in re-dissolving this sticky texture for analytical purposes. It's worth noting that while water does not interfere with HPLC, GC, NMR, or phenol-sulfuric acid assays or enzymatic assays, freeze-dried samples become highly hygroscopic, readily absorbing moisture from the surroundings. To address this issue, it is strongly recommended to use vacuum sealing before shipping. Additionally, informing the recipient about the hygroscopic nature of the sample is crucial for facilitating proper storage and handling.