Problem to dissolve 50%wt Glycerol in 4%wt PVOH 28-99 ??

Hello everyone, I am trying to prepare a solution out of PVA(Polyvinyl alcohol)_Glycerol_Wasser . (4% PVA 28-99 and 50% Glycerin) To prepare this solution I first add PVA to 80degree water and...

06 February 2021 2,300 3 View

Silane solution and application to Glass ?

30 January 2021 7,924 3 View

Flowcytometry after chemotaxis assay?

I did flowcytometry on the migrated t cells in chemotaxis assay to assess some surface markers. However the number of events is pretty low (less than 4000, aurora machine, high flow rate), which...

27 August 2020 1,023 3 View

I how to produce w1/o/w2 using silicone oil as o phase and that with different ratio of inner water to oil as well as different interfacial areas?

hi everyone, the challenge in my project is to produce double emulsion with exactly the same material system but with different ratios of inner water to oil (silicone oil as o phase)as well as...

22 July 2020 5,223 3 View

How can we define artificial intelligence, in biotechnology and chemical engineering projects?

How can we define artificial intelligence, in biotechnology and chemical engineering projects? I want to combine these two areas But I have no background in artificial intelligence

26 March 2020 5,370 1 View

Calcule the refractive index and extinction coefficient of multilayers?

hi, how can I calculate the equivalent refractive index and equivalent extinction coefficient of multilayers from the optical information of each layer, is there any simple software to calculate...

06 November 2019 5,507 6 View

How is Golgi staining procedure for Drosophila brain?

I look for a robust and simple protocol for Golgi staining of Drosophila CNS. Please share your experience in this matter with me.

01 October 2019 9,029 1 View

How to define PEDOT: PSS in Silvaco?

I found a problem to define an organic material which is PEDOT: PSS in silvaco , could anyone helps me out?

26 June 2019 7,483 1 View

Switched overlapping estimators technique and How we can run two estimators simultaneously but in an overlapping fashion?

Dear all, Please, i need a helpful example to examine the issue of how and when to update, or re-initialize, the computations with SIMULINK or Matlab. How we can run two estimators...

18 October 2018 6,868 3 View

How calculate the time consuming with Simulink ?

I want to calculate the computing time between two controllers implemented with Simulink. But when I try to use the "tic toc " function,. I got for the same controller two different values and are...

20 July 2018 690 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why the storage moduli E' decrease with highr fiber volume?

Hi guys, I am facing problems to interpret a DMA results. Material: Composite Bisphenol A + curaua fiber What is being tested: the influence of fiber volume on the composites properties...

12 February 2021 174 7 View

What can I do to keep magnetic beads suspended in solution within a syringe?

I'm looking for ways to prevent the settling of magnetic beads in solution. I have been using a syringe pump to inject a suspension of magnetic beads in PBS into a microfluidic device, and so far...

09 February 2021 6,206 2 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

Is it better to push or pull liquid through microfluidics with a syringe pump?

I have a PDMS microfluidic device that I want to perfuse cells through (there are also adherent cells in the channels). Is there any difference if I use a syringe pump to push the cells through...

30 January 2021 4,092 3 View

Is there any software to generate pdb of G quadruplex?

I have a DNA sequence which has predictability to form G quadruplex . I need a software to generate a pdb of it which will form G quadruplex. I am using open Babel but I am not able to set the...

29 January 2021 6,190 4 View

How can you attach polyurethane devices on glass slides?

I am making microfluidic devices made of polyurethane. The problem I am facing is that I am unable to attach these devices to glass slide even after plasma treatment (air plasma). Some of the...

28 January 2021 5,194 2 View

How can I measure speed of integration of an acquisition with secondary data?

I am currently faced with the challenge to measure integration speed of an acquisition or a merger with secondary data. Usually companies do not communicate once a target has been integrated...

27 January 2021 9,741 11 View

For DNA purification, what is the difference between purifying magnetic beads and column?

Hi! I noticed that mostly the single cell assay use AMPure beads (magnetic) instead of purifying column. But both function through a DNA affinity coating, just on surface in beads and on membrane...

27 January 2021 9,043 5 View

Can I use the Chlorophyll stability index (CSI) to calculate the stress potential of cyanobacteria that are under stress?

I want to calculate the stress potential of cyanobacterial cells that are inoculated with antagonistic bacteria. My results showed that there is a drastic reduction in the chlorophyll content of...

23 January 2021 331 3 View