I am investigating the influence of salinity on hump seed germination and need to establish a Complete Randomized Design (CRD) for conducting the germination tests. I have a couple of questions:
1. How can a Complete Randomized Design (CRD) be employed to evaluate the effect of salinity on hump seed germination?
2. Can the germination test be conducted on a germination plate, or is it necessary to use pots? Could someone provide a detailed explanation of the process?
As ferst step you can use Petri plate for germination test in lab.
First, you need to make sure all of the seeds are viable. You can use the flotation test for this (viable seeds will sink in water). Then you place viable seeds (at least 30) on each medium (pot or plate), using at least 6 of these (these are your subsamples). You then replicate this whole process at least 3 times, but each replicate must be distant from each other. Environmental conditions must be the same for all replicates and the pots/plates must be rotated within each replicate to ensure equal microclimate conditions. You perform the whole process with fresh water and repeat it with different salinity levels. The process is not strictly random, but it's not necessary for this type of experiment since it is replicated :)
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