Is it possible to detect T helper subsets with FACS without in vitro differentiation?

Hi all, I wonder if it is possible to distinguish mouse spleen Th1, Th2 and Th17 subsets based on surface markers with Flow Cytometry WITHOUT in vitro differentiation or maybe after a 2-3d...

31 December 2018 9,097 2 View

RNA extraction from low number cells?

Hi all. Any ideas about extracting RNA from low number (~1000-10000) mouse cells that have already been frozen with Trizol? I am aware of RNA kits but most of them provide their own buffers. My...

10 November 2017 7,872 4 View

Thymocytes isolation protocol?

04 May 2016 2,128 3 View

Best tool for finding dna binding sites between a known dna sequence and possible targets?

For all bioinformatic experts, I have a known sequence (DNA) and I'd like to find the DNA-binding consensus seq. between my known seq. of interest and possible others (unknown) targets. Which is...

09 October 2015 6,824 3 View

Has anybody used a proper ELISA kit that detects human Fibrinogen b in samples from human serum?

08 September 2015 8,190 3 View

How can I do a Competitive ELISA set up from scratch?

I'd like to perform a competitive ELISA from scratch. Being a first-timer, I would appreciate if someone could help me with the basic concetrations of Ab and antigen in oder to start with and...

03 April 2015 8,729 2 View

Has anyone used biotin tyramide for ELISA?

Hello! Has anybody used Biotin Tyramide (B-T) for signal amplification in an ELISA procedure? What was the optional concentration (at least a range)? Was it a ready made kit or home-made...

01 February 2015 1,255 3 View

How to increase the ELISA sensitivity?

I am performing ELISA for human TNF-alpha in serum samples (stored at -80oC). The Abs and recombinant protein (for standard curve) I use are purchased from eBioscience (paired match). I need a...

08 September 2014 5,501 14 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

03 March 2021 5,360 2 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View