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Questions related from Spyridoula Tsiomita
Any specific markers for murine gamma delta T cells for Flow Cytometry??
09 September 2019 4,696 4 View
Hi all, I wonder if it is possible to distinguish mouse spleen Th1, Th2 and Th17 subsets based on surface markers with Flow Cytometry WITHOUT in vitro differentiation or maybe after a 2-3d...
01 January 2019 9,138 2 View
Hi all. Any ideas about extracting RNA from low number (~1000-10000) mouse cells that have already been frozen with Trizol? I am aware of RNA kits but most of them provide their own buffers. My...
11 November 2017 7,920 4 View
Since I am new to working with mouse, could anyone be as kind as to provide a (detailed) protocol about thymocytes isolation from mice thymus? I have searched the web but I didn't find anything...
05 May 2016 2,169 3 View
For all bioinformatic experts, I have a known sequence (DNA) and I'd like to find the DNA-binding consensus seq. between my known seq. of interest and possible others (unknown) targets. Which is...
10 October 2015 6,890 3 View
Has anybody used a proper ELISA kit that detects human Fibrinogen b in samples from human serum? Thank you in advance.
09 September 2015 8,227 3 View
I'd like to perform a competitive ELISA from scratch. Being a first-timer, I would appreciate if someone could help me with the basic concetrations of Ab and antigen in oder to start with and...
04 April 2015 8,768 2 View
Hello! Has anybody used Biotin Tyramide (B-T) for signal amplification in an ELISA procedure? What was the optional concentration (at least a range)? Was it a ready made kit or home-made...
02 February 2015 1,296 3 View
I am performing ELISA for human TNF-alpha in serum samples (stored at -80oC). The Abs and recombinant protein (for standard curve) I use are purchased from eBioscience (paired match). I need a...
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