Markers for murine gamma delta T cells?

Any specific markers for murine gamma delta T cells for Flow Cytometry??

08 September 2019 4,637 4 View

Is it possible to detect T helper subsets with FACS without in vitro differentiation?

Hi all, I wonder if it is possible to distinguish mouse spleen Th1, Th2 and Th17 subsets based on surface markers with Flow Cytometry WITHOUT in vitro differentiation or maybe after a 2-3d...

31 December 2018 9,097 2 View

Thymocytes isolation protocol?

04 May 2016 2,128 3 View

Best tool for finding dna binding sites between a known dna sequence and possible targets?

For all bioinformatic experts, I have a known sequence (DNA) and I'd like to find the DNA-binding consensus seq. between my known seq. of interest and possible others (unknown) targets. Which is...

09 October 2015 6,824 3 View

Has anybody used a proper ELISA kit that detects human Fibrinogen b in samples from human serum?

08 September 2015 8,190 3 View

How can I do a Competitive ELISA set up from scratch?

I'd like to perform a competitive ELISA from scratch. Being a first-timer, I would appreciate if someone could help me with the basic concetrations of Ab and antigen in oder to start with and...

03 April 2015 8,729 2 View

Has anyone used biotin tyramide for ELISA?

Hello! Has anybody used Biotin Tyramide (B-T) for signal amplification in an ELISA procedure? What was the optional concentration (at least a range)? Was it a ready made kit or home-made...

01 February 2015 1,255 3 View

How to increase the ELISA sensitivity?

I am performing ELISA for human TNF-alpha in serum samples (stored at -80oC). The Abs and recombinant protein (for standard curve) I use are purchased from eBioscience (paired match). I need a...

08 September 2014 5,501 14 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View