How to deal with chance constraints in Matlab toolbox ga?

I am learning chance constraints. I want to know how to deal with chance constraints with generic algorithm, especially in matlab toolbox ga? Thank you a lot !

15 March 2020 3,472 3 View

Entropy is not a state variable, and there is a physical difference between it and internal energy?

Entropy is not a state variable, and there is a physical difference between it and internal energy. In the textbook, the ideal gas entropy: S = n * Cv * In (T) +nR * In (V); There are two...

01 April 2019 9,740 6 View

Is there a reasonable interdisciplinary argument for the second law of thermodynamics?

1. There are many kinds of type 2 perpetual motion machines. Behind each type is a natural phenomenon. Machine A: against the irreversibility of thermodynamics (diffusion, heat conduction,...

23 March 2019 7,786 4 View

Why the counterparts of virus A infectious clone swapped by counterparts of virus B cDNA fragments, virus A plasmid changed to low-copy?

does someone knows why the counterparts of virus A infectious clone swapped by same region of virus B cDNA fragments, virus A plasmid changed to low-copy?

14 January 2019 5,720 2 View

Why I can't see the unbonded free nucleotide probe in EMSA?

31 December 2018 2,342 5 View

What's this white precipitate after ammonium sulphate precipitation?

After adding 50% saturation of ammonium sulphate into a spent potato-dextrose liquid medium (300 mL), about 300 mg white precipitate showed up. Initially, I thought they should be proteins or...

11 June 2018 5,281 2 View

Tamoxifen treatment dose and time in mice?

Does anyone treat mice (B6) with tamoxifen to mimic ovarian ablation (ovariectomy)? How much and how long do you treat the mice? Thanks.

10 November 2016 2,788 2 View

ELISA for Glycated hemoglobin (HbA1c) measurement?

22 August 2016 1,369 4 View

How strong could actually the air contamination affect on Dirac point of GFET ?

24 July 2016 6,536 1 View

What are the main reasons for the formation of abnormally high content of smectite in the Qaidam Basin?

15 March 2016 5,400 9 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Build an orthogonal box in ASE?

Hello, I was wondering the best way to build an orthogonal box filled with a defined crystal structure in ASE. I know it could be easily done in LAMMPS, but I guess it is also doable in ASE. The...

21 February 2021 5,899 4 View

Does anyone know, can i do cryosectioning of whole aorta after oil red O staining and use the cryosection parts for further analysis?

I would like to quantify the lesions on aorta. I want to take image of atherosclerotic plaques formation in aorta by using O red Oil Solution. After the staining, what will be the storage...

16 February 2021 5,876 2 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

How to obtain regions around the person bounding boxes(excluded the detected people in scene)?

Hi all. I have a set of coordinates of bounding boxes for AVA Dataset. This dataset is used in action recognition. My goal is to get the areas around the people (people are inside boxes) and do...

13 February 2021 2,538 2 View