10 December 2020 3 9K Report

Hi, I used ExoQuick™Exosome Precipitation Solution to isolate exosomes from Plasma by following the standard protocol and resuspended the exosome pellet using 1X PBS buffer. I added NuPAGE™ LDS Sample Buffer (NP007) and NuPAGE Sample Reducing Agent (NP0009) to the solution and boiled at 90C for 5min to make sample for western blotting. However, the sample didn't run correctly. I found that two tracking dyes Coomassie G250 and Phenol Red didn't run in band shape, instead they lag behind compared with other samples and were smeared along the whole lane, some dyes still stack in wells.

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