This may seem like an unconventional inquiry. However, we're looking to conduct an initial biochemical study with a focus on identifying strong protein-protein interactions within mature human ribosomes. Specifically, we're curious if there's an effective technique for disassembling these ribosomes into smaller protein-RNA complexes.
If that's not feasible, would it be possible to first utilize a crosslinking agent to stabilize these interactions, and then apply a chaotropic agent for ribosomal denaturation? This would be followed by analysis through IP, de-crosslinking, and Western blotting. We understand that the details of this approach would need meticulous optimization to accurately capture our target interactions while minimizing disruption to the overall ribosomal structure.
Thank you for any suggestions you can offer!
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