I wondered how to examine the telomere length, especially in Drosophila melanogaster and is there a recommended detailed protocol for this work
thanks all for your input this question is well-answered
11 April 2024 2,661 3 View
In other words, why cannot the grain boundaries follow the high frequency ac signal?
13 March 2024 2,868 0 View
Materials include SnCl2, SbCl3, and citric acid.
21 February 2024 4,457 0 View
I am making electrodeposition liquid of tin antimony, and I want to dissolve 0.3M SnCl2·H2O and 0.03M SbCl3 in 0.3M citric acid aqueous solution, but it cannot be dissolved and white precipitate...
19 February 2024 7,614 1 View
We are currently attempting to create a Ti/Sb–SnO2 electrodeposition solution. While we have come across various methods in the literature, there is a lack of specific procedural details. This has...
12 November 2023 6,752 0 View
I want to prepare a DES system for electrodeposition, but I've been unable to do so. Choline Chloride: Ethylene Glycol in a 1:2 ratio. When heated in an 80°C water bath, the resulting transparent...
24 October 2023 3,647 0 View
Hi all, Does lumen changes in a light box or transilluminator uesd for plaque assay would have great impact on enumeration? Or maybe there is a opportune range of lumen for these equippments when...
29 August 2023 3,322 0 View
This may seem like an unconventional inquiry. However, we're looking to conduct an initial biochemical study with a focus on identifying strong protein-protein interactions within mature human...
27 August 2023 4,152 0 View
I am experiencing issues with the expression of phosphorylated proteins in my western blot experiments. Specifically, I observe strong phosphorylated protein expression but no expression of the...
21 June 2023 3,688 3 View
I am dealing with a crack simulation using ANSYS APDL mechanical. For some purpose, I need to extract the location of crack tip under every substep of simulation. The simulation was done by 2D...
25 April 2023 2,416 1 View
Dear all, I have performed CUT&TAG and tried to pulldown H3K27me3 containing nucleosomes in the cells treated with EZH2 inhibitor. I spiked my human cells with 20% of Drosophila cells. As...
21 April 2024 3,197 0 View
Hello team, I am using Mirdeep2 to identify miRNA in my organism (none model organism) which has no previously identified micro_RNAs. My organism is genetically close to Drosophila melanogaster....
14 April 2024 9,090 0 View
Hi all, I am using HA beads to do a Co-IP (using drosophila whole body), then do mass spec to detect the interacting partners that pulled down by HA. The one that I tried before was not good, it...
09 April 2024 2,374 0 View
Hello, Apologies if there's an obvious answer to this, I'm a first year PhD student and this task is entirely new to me. I'm including a lot of detail to convey my question clearly. In D....
04 April 2024 9,635 3 View
behavioural study of Drosophila melanogaster in DD conditions
08 February 2024 2,608 0 View
What kind of software are you using to analyze courtship behavior in Drosophila?
22 December 2023 7,302 0 View
I need a detailed protocol of SDS-PAGE only for drosophila if anyone has done before
16 November 2023 7,784 1 View
When using the UAS-GAL4 system to express the transgene in a fly (at the white prepupa stage), does altering the temperature from 25C to 18C reduce the effciency of the GAL4 driver in turn leading...
15 October 2023 5,416 4 View
The Tm of primers is 65 degrees and I am getting only non specific bands around 1kb. I amplified using Q5-HF Polymerase. How can I improve the specificity to get my desired band?
17 September 2023 4,956 5 View
I have an unusual question: I am working on a Erasmus internship project with Drosophila mutants at 2 different timepoints and with WT, KO and KI condition. A company analyzed the data using...
11 September 2023 1,743 1 View