Luciferase assay for miRNA-gene: How to obtain DNA (PCR product) from the mutated primer?

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Despite single-cell RNA, how can I characterize a new cell subtype/phenotype?

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Permanent Fluid temperature initialization in Ansys Fluent?

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How to couple Moldex 3D with MATLAB for design optimization of an injection mould?

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How to perform Potential Surface Energy Exploration (PES) calculations?

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How to get the model shape matrix from Nastran?

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How to compare two groups with only two measurements?

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Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

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What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

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How to increase a model accuracy ?

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In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

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Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

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Sample dilution for quantitative analysis using ELISA?

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Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

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Andreas Eisenreich

The mutation can be inserted via the corresponding side-directed mutaion assay kit. I would propose to check the manufacturers protocoll for such a kit.

Keteryne Silva

Hi @Andreas Eisenreich

Thanks to reply!

I didn't know I could use a kit to perform that. I designed all my primers (WT and mutated), is there any way to perform the assay without this kit? 'Cause I've checked some papers and not all of them say that they used a Kit for it.

Ali Malik Saad

You can use kit or other techniques.