Keteryne Silva

3 Questions 4 Answers 0 Followers

Questions related from Keteryne Silva

Besides the MUTZ-3, does anyone know any immortalized human osteoclast cell line?

I would like to obtain an immortalized human osteoclast cell line.

09 January 2023 9,424 1 View

Luciferase assay for miRNA-gene: How to obtain DNA (PCR product) from the mutated primer?

To perform the Luciferase assay for microRNA-gene interaction its necessary a primer (wild-type) to amplificate the specific ligation site and also a mutated primer. But if your primer has a...

20 January 2021 8,785 3 View

Luciferase assay for miRNA-gene: How to construct the mutated prime?

I have experimental evidence (qRT-PCR and WB) to suggest a miRNA downregulates a gene. To test if the regulation is direct I want to perform a luciferase reporter assay. So I designed a primer...

16 October 2020 7,750 4 View