Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

Tapestation vs Agarose gel following PCR reaction - what are the extra bands?

We recently have been moving away from using agarose gels and onto using the Tapestation (D1000 for PCR products). However, we have noticed that there are a lot of extra bands that show up on the...

15 February 2021 791 3 View

How to quantify how much of a fluorescent dye is entering a cell?

I'm looking to compare a few fluorescent dyes for cell permeability. I'm interested to see how much of the dye actually enters the cell, but would specifically like to quantify this data. Any...

21 January 2021 6,089 3 View

How to increase presynaptic neurotransmitter release artificially?

I would like to pharmacologically increase presynaptic neurotransmitter release in mice CNS in vivo (through the extracellular micro-infusion of a compound). Which strategy would you recommand?...

02 November 2020 5,645 3 View

Why does my plasmid grow when transformed into DH5a competent cells but not BL21?

I have a custom plasmid which expresses mCherry and will act as a reporter. I can transform this plasmid into DH5a and BL21. With DH5a I get a good number of singular colonies, but with BL21 I get...

02 September 2020 9,063 3 View

Does anyone uses casein-TBS buffer (1%) for blocking of fluorescence blots?

Hi there, we try to establish a multiplex western blotting method using fluorescence-conjugated sec. antibodies (anti-rabbit IgG StarBright Blue 520 and anti-mouse IgG StarBright Blue 700 from...

27 August 2020 7,787 5 View

Recommended measures to investigate change in psychological flexibility - ACT/CBS?

Hi All, I am looking for recommended valid measures/questionnaires to measure a change in psychological flexibility. To be delivered pre and post intervention. Can either measure psychological...

18 August 2020 8,187 3 View

Is there any statistical test i can perform on fold changes between cell lines to determine significance in protein expression values?

Hi there! I am currently writing up my MSc oncology dissertation; I have 5 cancer cell lines, 1 responds to a certain treatment, the other 4 do not. I am trying to find a statistical test which...

09 July 2020 3,720 4 View

How to interpret coefficient in regression when the independent variable is a change in %?

Good morning all, I am having some trouble interpreting my regression output. My model is LN of transaction volume = alpha + beta1_RER + control variables RER is defined as the change in the...

28 April 2019 516 3 View

How do you interpret Likert-scale answers to 15 questions, comparing two samples?

Part of my dissertation looks at a replication of the NEP study conducted in 2000. I'm attempting to compare the two (my repsonses and the responses received in 2000) and see whether my survey...

06 March 2019 4,050 6 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View