Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

Tapestation vs Agarose gel following PCR reaction - what are the extra bands?

We recently have been moving away from using agarose gels and onto using the Tapestation (D1000 for PCR products). However, we have noticed that there are a lot of extra bands that show up on the...

15 February 2021 791 3 View

How to quantify how much of a fluorescent dye is entering a cell?

I'm looking to compare a few fluorescent dyes for cell permeability. I'm interested to see how much of the dye actually enters the cell, but would specifically like to quantify this data. Any...

21 January 2021 6,089 3 View

How to increase presynaptic neurotransmitter release artificially?

I would like to pharmacologically increase presynaptic neurotransmitter release in mice CNS in vivo (through the extracellular micro-infusion of a compound). Which strategy would you recommand?...

02 November 2020 5,645 3 View

Why does my plasmid grow when transformed into DH5a competent cells but not BL21?

I have a custom plasmid which expresses mCherry and will act as a reporter. I can transform this plasmid into DH5a and BL21. With DH5a I get a good number of singular colonies, but with BL21 I get...

02 September 2020 9,063 3 View

Does anyone uses casein-TBS buffer (1%) for blocking of fluorescence blots?

Hi there, we try to establish a multiplex western blotting method using fluorescence-conjugated sec. antibodies (anti-rabbit IgG StarBright Blue 520 and anti-mouse IgG StarBright Blue 700 from...

27 August 2020 7,787 5 View

Recommended measures to investigate change in psychological flexibility - ACT/CBS?

Hi All, I am looking for recommended valid measures/questionnaires to measure a change in psychological flexibility. To be delivered pre and post intervention. Can either measure psychological...

18 August 2020 8,187 3 View

Is there any statistical test i can perform on fold changes between cell lines to determine significance in protein expression values?

Hi there! I am currently writing up my MSc oncology dissertation; I have 5 cancer cell lines, 1 responds to a certain treatment, the other 4 do not. I am trying to find a statistical test which...

09 July 2020 3,720 4 View

How to interpret coefficient in regression when the independent variable is a change in %?

Good morning all, I am having some trouble interpreting my regression output. My model is LN of transaction volume = alpha + beta1_RER + control variables RER is defined as the change in the...

28 April 2019 516 3 View

How do you interpret Likert-scale answers to 15 questions, comparing two samples?

Part of my dissertation looks at a replication of the NEP study conducted in 2000. I'm attempting to compare the two (my repsonses and the responses received in 2000) and see whether my survey...

06 March 2019 4,050 6 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

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02 March 2021 3,060 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View