I'm extracting plasmid DNA with 'insert ' from but nonodrop quantification has detected the conc. of plasmid DNA to be
With a low concentration, it is important to know the quality. What is your 260/280 ratio? A ratio between 1.8 and 2.0 is ideal, and in that case your plasmid should be fine for PCR. If your 260/280 is lower or higher than the given range, you may want to further purify (phenol chloroform extraction, ethanol precipitation). For more info on 260/280 and its relation to purity, see http://bitesizebio.com/13532/how-measurement-of-concentration-and-purity-of-nucleic-acids-works/
Thankyou Eric.
My 260/280 read for most of my clones is with in 1.37-2.13 and conc. of 16.3-98.6ng/µl, the majority being with in 1.8-2.0 absorbance value. I'm worried to use these clones (plasmid DNA) for PCR. So you are advicing me to use all clones falling with in 1.8-2.0 OD though their con. is less than 100ng/µl (say 20-100)?
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