Anyone to tell me how to construct phylogenetic tree from distance matrix using mega 7?

Rather than using sequence alignment data, I wanted to have phylogenetic tree from distance matrix and bootstrap as part of statistical analysis. Anyone to tell me how to execute this analysis?

02 March 2017 8,205 4 View

Anyone with exprience with BAC insert sequencing?

Any strategy to cut BAC insert into smaller fragments so that I can sequence the fragments using Sanger sequencer (3130x/Genetic Analyzer)? I would be happy to know reagents required.

05 June 2016 3,090 1 View

Anyone with exprience in working with large BAC library multiplication using 384 well plate multiplicator system?

03 April 2016 8,555 2 View

Anyone having BAC clone duplicator apparatus (equipment) usage expriences?

I am going to start a BAC clone multiplication. There is about 58600 clones kept at -80 degree C. in 384 well plate. I am searching for some 7 genes of my interest whereby I group clones and...

02 March 2016 459 0 View

Can anyone help on RT-PCR background/baseline calibration on Applied Biosystems PCR system?

I have run RT-PCR plate to test two primers on two diffrent cDNA (both at conc. of 500ng/microlitre) samples taken at diffrent times. The same concentration of primers (2.5micromolar) resulted...

11 December 2015 3,930 5 View

Which primer is preferable for sequencing: M13 (-21) or another one?

I have a question in choosing primers for sequencing. Which one is preferable: using only M13 (-21) or sp6 and T7 forward and reverse primers, respectively? As I don't know the orientation of my...

09 October 2014 270 6 View

What is the advantage of colony pcr over miniprep plasmid extraction to confirm the insert into a vector?

What are the main advantages of colony PCR over plasmid miniprep procedures to confirm the ligation of an insert into a plasmid vector?

09 October 2014 1,903 15 View

What is the conc. (and purity of PCR product at 260/280nm) that could be used for sequencing?

What is the conc. (and purity of PCR products at 260/280nm) that could be used for sequencing. I have DNA samples amplified by PCR and thinking to use directly for sequencing than using the...

09 October 2014 5,799 16 View

No title

08 September 2014 2,626 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View