Geleta Dugassa Barka

10 Questions 14 Answers 0 Followers

Questions related from Geleta Dugassa Barka

Anyone to tell me how to construct phylogenetic tree from distance matrix using mega 7?

Rather than using sequence alignment data, I wanted to have phylogenetic tree from distance matrix and bootstrap as part of statistical analysis. Anyone to tell me how to execute this analysis?

03 March 2017 8,269 4 View

Anyone to recommend RT-qPCR analysis software?

I am looking for a candidate RT-qPCR analysis software complying MIQE requirements. Most softwares for RTqPCR anlysis simply do the normalization with reference genes and refernce samples with...

07 July 2016 2,031 2 View

Anyone with exprience with BAC insert sequencing?

Any strategy to cut BAC insert into smaller fragments so that I can sequence the fragments using Sanger sequencer (3130x/Genetic Analyzer)? I would be happy to know reagents required.

06 June 2016 3,150 1 View

Anyone with exprience in working with large BAC library multiplication using 384 well plate multiplicator system?

While using the 384 well plate replicator, what is the means of sterilization (disinfection) between plates to pick new clones from one plate to another? Anyone with experience on large BAC...

04 April 2016 8,632 2 View

Anyone having BAC clone duplicator apparatus (equipment) usage expriences?

I am going to start a BAC clone multiplication. There is about 58600 clones kept at -80 degree C. in 384 well plate. I am searching for some 7 genes of my interest whereby I group clones and...

03 March 2016 552 0 View

Can anyone help on RT-PCR background/baseline calibration on Applied Biosystems PCR system?

I have run RT-PCR plate to test two primers on two diffrent cDNA (both at conc. of 500ng/microlitre) samples taken at diffrent times. The same concentration of primers (2.5micromolar) resulted...

12 December 2015 3,992 5 View

What is the conc. (and purity of PCR product at 260/280nm) that could be used for sequencing?

What is the conc. (and purity of PCR products at 260/280nm) that could be used for sequencing. I have DNA samples amplified by PCR and thinking to use directly for sequencing than using the...

10 October 2014 5,869 16 View

Which primer is preferable for sequencing: M13 (-21) or another one?

I have a question in choosing primers for sequencing. Which one is preferable: using only M13 (-21) or sp6 and T7 forward and reverse primers, respectively? As I don't know the orientation of my...

10 October 2014 359 6 View

What is the advantage of colony pcr over miniprep plasmid extraction to confirm the insert into a vector?

What are the main advantages of colony PCR over plasmid miniprep procedures to confirm the ligation of an insert into a plasmid vector?

10 October 2014 2,120 15 View

I'm extracting plasmid DNA with 'insert ' from but nonodrop quantification has detected the conc. of plasmid DNA to be <100ng/µl. Is it ok for PCR?

I'm extracting plasmid DNA with 'insert ' from but nonodrop quantification has detected the conc. of plasmid DNA to be

09 September 2014 2,686 2 View