Anyone to tell me how to construct phylogenetic tree from distance matrix using mega 7?

Rather than using sequence alignment data, I wanted to have phylogenetic tree from distance matrix and bootstrap as part of statistical analysis. Anyone to tell me how to execute this analysis?

02 March 2017 8,205 4 View

Anyone to recommend RT-qPCR analysis software?

I am looking for a candidate RT-qPCR analysis software complying MIQE requirements. Most softwares for RTqPCR anlysis simply do the normalization with reference genes and refernce samples with...

06 July 2016 1,942 2 View

Anyone with exprience in working with large BAC library multiplication using 384 well plate multiplicator system?

03 April 2016 8,555 2 View

Anyone having BAC clone duplicator apparatus (equipment) usage expriences?

I am going to start a BAC clone multiplication. There is about 58600 clones kept at -80 degree C. in 384 well plate. I am searching for some 7 genes of my interest whereby I group clones and...

02 March 2016 459 0 View

Can anyone help on RT-PCR background/baseline calibration on Applied Biosystems PCR system?

I have run RT-PCR plate to test two primers on two diffrent cDNA (both at conc. of 500ng/microlitre) samples taken at diffrent times. The same concentration of primers (2.5micromolar) resulted...

11 December 2015 3,930 5 View

Which primer is preferable for sequencing: M13 (-21) or another one?

I have a question in choosing primers for sequencing. Which one is preferable: using only M13 (-21) or sp6 and T7 forward and reverse primers, respectively? As I don't know the orientation of my...

09 October 2014 270 6 View

What is the advantage of colony pcr over miniprep plasmid extraction to confirm the insert into a vector?

What are the main advantages of colony PCR over plasmid miniprep procedures to confirm the ligation of an insert into a plasmid vector?

09 October 2014 1,903 15 View

What is the conc. (and purity of PCR product at 260/280nm) that could be used for sequencing?

What is the conc. (and purity of PCR products at 260/280nm) that could be used for sequencing. I have DNA samples amplified by PCR and thinking to use directly for sequencing than using the...

09 October 2014 5,799 16 View

No title

08 September 2014 2,626 2 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is there any Machine Learning/AI algorithm that lets you predict with incomplete features without missing value imputation process?

For instance, the model is trained with A,B,C,D,E features to predict F target. Then, I want to make prediction on a new data, but with only A,C,D,E is known without doing missing value imputation...

28 February 2021 1,556 6 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View