Rather than using sequence alignment data, I wanted to have phylogenetic tree from distance matrix and bootstrap as part of statistical analysis. Anyone to tell me how to execute this analysis?
Dear Geleta,
Please use in http://www.genome.jp/tools/clustalw/ to learn step by step how to move from alignment to phylogenetic tree. Best Regards
Hello Andrey, thank you for the site. However, my main question is how can I take my distance matrix to mega 7 and construct phylogenic tree considering bootstrap statistical method. The distance matrix I have is developed from markers.
Not sure if you mean that you already have the distance matrix of your sequences or whatever and you need to simulated that values by bootstrap (as the statistical analysis) in MEGA for your distance phylogenetic tree without the sequence data ? Anyway, I have no idea how to do.
I understand from your question that you already have matrix distance for your sequences, but why dont try to align your sequences in MEGA then choose the approach you need to construct your phylogenetic tree?. We need to know your matrix distance in which format and in which program you created?, and maybe can find the way to edit in MEGA then you can construct your phylogenetic tree.
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