Hi Everyone,
How can I separate Angiotensinogen or its peptides from Human serum?
Hi Everyone, I am working on tetra-ARMS-PCR to genotype the rs6387. The size of amplicons is: control 610bp, A allele 281bp and G allele 382bp. The PCR is showing a perfect amplification with...
11 December 2018 8,121 3 View
I am working on mice aorta and I am new to microscopy. I have prepared slide of mice aorta sections and imaged under light microscope (I know its not very good for analysis of aorta sections)....
07 August 2018 7,355 1 View
I have data of drug response (0,1) and genotypes (1,2,3). in genotypes, some catagories have count less than 5. Fisher exact test cannot be applied as the data is 2X3. Which other test will be...
07 August 2018 2,356 9 View
Hi everyone, I want to extract total proteins from chicken heart and liver tissues. please suggest any simple method with least number of chemicals. Please also explain the composition of the...
09 October 2017 9,011 1 View
Hi, I extracted DNA from blood samples by organic method with freshly prepared solutions. Dissolved DNA in double distiled deionized water (Like I did for my previous samples). see figure 1 for...
08 September 2017 9,490 7 View
Hi everyone, I am working on case control genetic data. I have data for SNP with genotypes AA, AG and GG. I want to check the effect of these individual genotypes on the disease outcome, which...
07 August 2017 9,812 4 View
Hi, I am working on a project related to cardiovascular diseases. I just started my sampling. I take blood sample in Gel coated vaccutainer as I need serum to perform biochemical tests by myself...
02 March 2016 5,015 7 View
I want to derivatize my fatty acids in methyl esters. according to protocol I should add 10% BF3/methanol in the sample. I want to confirm that does 10% BF3/methanl means 10 ml BF3 in 90ml...
01 February 2016 2,613 3 View
Today I was extracted DNA from whole blood. after digestion of proteins (by using proteinase K and 10%SDS, gave incubation of 3 hours at 65 degree) I added Phenol:chloroform:isoamylalchohol which...
11 December 2015 4,511 2 View
Hi Everyone I want to check levels of renin, angiotensinogen and ACE in human serum samples of cardiac patients. Its first time I am going to perform ELISA. Can you suggest me few good kits? or...
01 January 1970 9,339 3 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...
30 July 2024 1,040 1 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View