Need help with Tetra ARMS PCR results and sequencing results?

Hi Everyone, I am working on tetra-ARMS-PCR to genotype the rs6387. The size of amplicons is: control 610bp, A allele 281bp and G allele 382bp. The PCR is showing a perfect amplification with...

11 December 2018 8,042 3 View

Chi square test, if data violates chi square assumptions?

I have data of drug response (0,1) and genotypes (1,2,3). in genotypes, some catagories have count less than 5. Fisher exact test cannot be applied as the data is 2X3. Which other test will be...

07 August 2018 2,281 9 View

Request for help with histology analysis?

I am working on mice aorta and I am new to microscopy. I have prepared slide of mice aorta sections and imaged under light microscope (I know its not very good for analysis of aorta sections)....

07 August 2018 7,290 1 View

How to extract proteins from chicken heart and liver tissues??

09 October 2017 8,932 1 View

Good genomic DNA but No PCR?

08 September 2017 9,418 7 View

Dominant, recessive and additive model in SPSS?

07 August 2017 9,689 4 View

Can I store serum at -30 degree for 1 or 2 days before performing biochemical tests?

02 March 2016 4,937 7 View

Preparation of 10% BF3/methanol?

01 February 2016 2,453 3 View

Why did my sample solution (during DNA Extraction) turn Milky White on addition of Phenol chloroform isoamyl alcohol?

11 December 2015 4,431 2 View

ELISA kits for Human Angiotensinogen, Renin and Angiotensin Converting Enzyme (ACE) ?

01 January 1970 9,272 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View