Need help with Tetra ARMS PCR results and sequencing results?

Hi Everyone, I am working on tetra-ARMS-PCR to genotype the rs6387. The size of amplicons is: control 610bp, A allele 281bp and G allele 382bp. The PCR is showing a perfect amplification with...

11 December 2018 8,042 3 View

Chi square test, if data violates chi square assumptions?

I have data of drug response (0,1) and genotypes (1,2,3). in genotypes, some catagories have count less than 5. Fisher exact test cannot be applied as the data is 2X3. Which other test will be...

07 August 2018 2,281 9 View

Request for help with histology analysis?

I am working on mice aorta and I am new to microscopy. I have prepared slide of mice aorta sections and imaged under light microscope (I know its not very good for analysis of aorta sections)....

07 August 2018 7,290 1 View

Good genomic DNA but No PCR?

08 September 2017 9,418 7 View

Dominant, recessive and additive model in SPSS?

07 August 2017 9,689 4 View

Isolation of Angiotensin peptides from Human serum?

10 November 2016 7,067 0 View

Can I store serum at -30 degree for 1 or 2 days before performing biochemical tests?

02 March 2016 4,937 7 View

Preparation of 10% BF3/methanol?

01 February 2016 2,453 3 View

Why did my sample solution (during DNA Extraction) turn Milky White on addition of Phenol chloroform isoamyl alcohol?

11 December 2015 4,431 2 View

ELISA kits for Human Angiotensinogen, Renin and Angiotensin Converting Enzyme (ACE) ?

01 January 1970 9,272 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View