Misbah Hussain

11 Questions 12 Answers 0 Followers

Questions related from Misbah Hussain

Need help with Tetra ARMS PCR results and sequencing results?

Hi Everyone, I am working on tetra-ARMS-PCR to genotype the rs6387. The size of amplicons is: control 610bp, A allele 281bp and G allele 382bp. The PCR is showing a perfect amplification with...

12 December 2018 8,085 3 View

Request for help with histology analysis?

I am working on mice aorta and I am new to microscopy. I have prepared slide of mice aorta sections and imaged under light microscope (I know its not very good for analysis of aorta sections)....

08 August 2018 7,329 1 View

Chi square test, if data violates chi square assumptions?

I have data of drug response (0,1) and genotypes (1,2,3). in genotypes, some catagories have count less than 5. Fisher exact test cannot be applied as the data is 2X3. Which other test will be...

08 August 2018 2,324 9 View

How to extract proteins from chicken heart and liver tissues??

Hi everyone, I want to extract total proteins from chicken heart and liver tissues. please suggest any simple method with least number of chemicals. Please also explain the composition of the...

10 October 2017 8,976 1 View

Good genomic DNA but No PCR?

Hi, I extracted DNA from blood samples by organic method with freshly prepared solutions. Dissolved DNA in double distiled deionized water (Like I did for my previous samples). see figure 1 for...

09 September 2017 9,463 7 View

Dominant, recessive and additive model in SPSS?

Hi everyone, I am working on case control genetic data. I have data for SNP with genotypes AA, AG and GG. I want to check the effect of these individual genotypes on the disease outcome, which...

08 August 2017 9,767 4 View

Isolation of Angiotensin peptides from Human serum?

Hi Everyone, How can I separate Angiotensinogen or its peptides from Human serum?

11 November 2016 7,105 0 View

Can I store serum at -30 degree for 1 or 2 days before performing biochemical tests?

Hi, I am working on a project related to cardiovascular diseases. I just started my sampling. I take blood sample in Gel coated vaccutainer as I need serum to perform biochemical tests by myself...

03 March 2016 4,991 7 View

Preparation of 10% BF3/methanol?

I want to derivatize my fatty acids in methyl esters. according to protocol I should add 10% BF3/methanol in the sample. I want to confirm that does 10% BF3/methanl means 10 ml BF3 in 90ml...

02 February 2016 2,555 3 View

Why did my sample solution (during DNA Extraction) turn Milky White on addition of Phenol chloroform isoamyl alcohol?

Today I was extracted DNA from whole blood. after digestion of proteins (by using proteinase K and 10%SDS, gave incubation of 3 hours at 65 degree) I added Phenol:chloroform:isoamylalchohol which...

12 December 2015 4,478 2 View

ELISA kits for Human Angiotensinogen, Renin and Angiotensin Converting Enzyme (ACE) ?

Hi Everyone I want to check levels of renin, angiotensinogen and ACE in human serum samples of cardiac patients. Its first time I am going to perform ELISA. Can you suggest me few good kits? or...

01 January 1970 9,313 3 View