Hi Everyone
I want to check levels of renin, angiotensinogen and ACE in human serum samples of cardiac patients.
Its first time I am going to perform ELISA. Can you suggest me few good kits? or what points I should keep in mind while choosing kits?
Hello, Here is the answer: ELISA for renin and ACE from R&D Systems, and ELISA for angiotensinogen from RayBiotech. Regards, Jan
@Simoni Thank you for your suggestions. These are really good kits with minimum CV% and other comparably better features.
@Lizzette Sanchez
Thank you so much for posting links. They helped me alot.
Hi Everyone, I am working on tetra-ARMS-PCR to genotype the rs6387. The size of amplicons is: control 610bp, A allele 281bp and G allele 382bp. The PCR is showing a perfect amplification with...
11 December 2018 8,121 3 View
I am working on mice aorta and I am new to microscopy. I have prepared slide of mice aorta sections and imaged under light microscope (I know its not very good for analysis of aorta sections)....
07 August 2018 7,355 1 View
I have data of drug response (0,1) and genotypes (1,2,3). in genotypes, some catagories have count less than 5. Fisher exact test cannot be applied as the data is 2X3. Which other test will be...
07 August 2018 2,356 9 View
Hi everyone, I want to extract total proteins from chicken heart and liver tissues. please suggest any simple method with least number of chemicals. Please also explain the composition of the...
09 October 2017 9,011 1 View
Hi, I extracted DNA from blood samples by organic method with freshly prepared solutions. Dissolved DNA in double distiled deionized water (Like I did for my previous samples). see figure 1 for...
08 September 2017 9,490 7 View
Hi everyone, I am working on case control genetic data. I have data for SNP with genotypes AA, AG and GG. I want to check the effect of these individual genotypes on the disease outcome, which...
07 August 2017 9,812 4 View
Hi Everyone, How can I separate Angiotensinogen or its peptides from Human serum?
10 November 2016 7,139 0 View
Hi, I am working on a project related to cardiovascular diseases. I just started my sampling. I take blood sample in Gel coated vaccutainer as I need serum to perform biochemical tests by myself...
02 March 2016 5,015 7 View
I want to derivatize my fatty acids in methyl esters. according to protocol I should add 10% BF3/methanol in the sample. I want to confirm that does 10% BF3/methanl means 10 ml BF3 in 90ml...
01 February 2016 2,613 3 View
Today I was extracted DNA from whole blood. after digestion of proteins (by using proteinase K and 10%SDS, gave incubation of 3 hours at 65 degree) I added Phenol:chloroform:isoamylalchohol which...
11 December 2015 4,511 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View