I am working on mice aorta and I am new to microscopy. I have prepared slide of mice aorta sections and imaged under light microscope (I know its not very good for analysis of aorta sections). Image is attached.
Can anyone please help me with analysis of this image? what Information I can get from this image?
Dear Misbah.
So you killed some mice, prepared aorta slices and you don´t know why you did that??
It would be interesting to mention what your aim is. What do you expect to see? Is this a stained slide? FFPE, or frozen?
If you still work in the hypertension field (as I assume after visiting your profile site), then you could look for aorta thickness using the ImageJ tool. For this you should record a better image without the grid and the lines and with a magnification bar.
Maybe a PubMed search could provide some ideas: What other scientists looked at and what methods they used.
Please provide more information in order to help you out.
Kind regards