Precipitates in BL buffer (Epitect DNA bisulfite Kit)?

01 February 2020 2,558 1 View

Isolation of total RNA from small pieces of tissue?

Hi Last time I got really small pieces of tissue from the hospital. There are 3 small pieces of stomach tissue in RNA later (1 mm) and I want to extract RNA by using MIRVANA isolation kit. Is it...

02 March 2019 3,651 3 View

Homogenisation of tissue by Tissuelyser II?

09 October 2018 8,164 2 View

Low absorption 260/230 in RNA-important for taqmanRNA?

Hi I have adsorption 260/230 in RNA samples betwen 2,1-1,3 (adsorption 260/280 is really good). How important is this information for my cDNA synstesis and taqman analyses. Can I use this samples?...

03 April 2018 5,921 1 View

Qubit microRNA Assay Kit for detecting in TotalRNA?

Hi everybody One question about Qubit microRNA Assay Kit. I isolated total RNA with using Mirvana microRNA isolation kit. Can i use Qubit mirna assay to detecting concentration of miRNA in total...

02 March 2018 5,874 2 View

Protocol for isolatio of Total RNA or small rna enrichment (Mirvana microRNA kit) in application in taqman mirna expression??

Hi I have a question about protocol of isolation RNA by Mirvana microRNA kit. I want to detect expression mirna in rats tissue of mammary gland tumor using of Taqman mirna. Is it correct use total...

02 March 2018 5,857 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View