Hi
Last time I got really small pieces of tissue from the hospital. There are 3 small pieces of stomach tissue in RNA later (1 mm) and I want to extract RNA by using MIRVANA isolation kit. Is it possible to extract RNA from so small tissue??
Thanks
Sure, this depends on the efficacy of your isolation procedure. Maybe you should use a Trizol(R) isolation method instead of a collumn-based isolation kit. This can increase your yield from (maybe too) small pieces of tissue.
pretty small tissue input, but should work, there are no better options. spin column based mirVana or magnetic bead-based MagMax mirVana, both kits efficiently isolate total RNA (including miRNA) from misc sample types.
I agree to some extent with Andreas. Sometimes you can get better RNA yield from using a Trizol-based method rather than spin columns. I know it's a pain compared to the columns, but it may be worth trying. If you're doing Trizol, I would also recommend adding glycogen in the ethanol precipitation step (0.5-1uL / sample works well for me, I use Invitrogen) which helps pellet RNA and genetic material. If your downstream application is RT-PCR, I would highly recommend using DNase to get rid of genomic DNA contamination. I use NEB DNase I, incubate according to their protocol for ~30 minutes and it almost virtually gets rid of all genomic DNA background noise from my samples.
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