Precipitates in BL buffer (Epitect DNA bisulfite Kit)?

01 February 2020 2,558 1 View

Isolation of total RNA from small pieces of tissue?

Hi Last time I got really small pieces of tissue from the hospital. There are 3 small pieces of stomach tissue in RNA later (1 mm) and I want to extract RNA by using MIRVANA isolation kit. Is it...

02 March 2019 3,651 3 View

No title

10 November 2018 4,507 0 View

Low absorption 260/230 in RNA-important for taqmanRNA?

Hi I have adsorption 260/230 in RNA samples betwen 2,1-1,3 (adsorption 260/280 is really good). How important is this information for my cDNA synstesis and taqman analyses. Can I use this samples?...

03 April 2018 5,921 1 View

Qubit microRNA Assay Kit for detecting in TotalRNA?

Hi everybody One question about Qubit microRNA Assay Kit. I isolated total RNA with using Mirvana microRNA isolation kit. Can i use Qubit mirna assay to detecting concentration of miRNA in total...

02 March 2018 5,874 2 View

Protocol for isolatio of Total RNA or small rna enrichment (Mirvana microRNA kit) in application in taqman mirna expression??

Hi I have a question about protocol of isolation RNA by Mirvana microRNA kit. I want to detect expression mirna in rats tissue of mammary gland tumor using of Taqman mirna. Is it correct use total...

02 March 2018 5,857 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View