Precipitates in BL buffer (Epitect DNA bisulfite Kit)?

01 February 2020 2,558 1 View

Isolation of total RNA from small pieces of tissue?

Hi Last time I got really small pieces of tissue from the hospital. There are 3 small pieces of stomach tissue in RNA later (1 mm) and I want to extract RNA by using MIRVANA isolation kit. Is it...

02 March 2019 3,651 3 View

No title

10 November 2018 4,507 0 View

Homogenisation of tissue by Tissuelyser II?

09 October 2018 8,164 2 View

Low absorption 260/230 in RNA-important for taqmanRNA?

Hi I have adsorption 260/230 in RNA samples betwen 2,1-1,3 (adsorption 260/280 is really good). How important is this information for my cDNA synstesis and taqman analyses. Can I use this samples?...

03 April 2018 5,921 1 View

Protocol for isolatio of Total RNA or small rna enrichment (Mirvana microRNA kit) in application in taqman mirna expression??

Hi I have a question about protocol of isolation RNA by Mirvana microRNA kit. I want to detect expression mirna in rats tissue of mammary gland tumor using of Taqman mirna. Is it correct use total...

02 March 2018 5,857 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View