Marek Samec

7 Questions 4 Answers 0 Followers

Questions related from Marek Samec

Precipitates in BL buffer (Epitect DNA bisulfite Kit)?

Has anyone experiences with the Qiagen Epitect DNA bisulfite Kit and with precipitates in BL buffer? I incubated a flask of BL at 60°C for 3 hours, and precipitates are still there. I found...

02 February 2020 2,620 1 View

Isolation of total RNA from small pieces of tissue?

Hi Last time I got really small pieces of tissue from the hospital. There are 3 small pieces of stomach tissue in RNA later (1 mm) and I want to extract RNA by using MIRVANA isolation kit. Is it...

03 March 2019 3,702 3 View

Isolation mirna from fresh/frozen tissue in -30°C?

Hi I would like to isolate miRNA from the tissue that is stored in -30°C. The tissue is in tubes without any RNAlater solution. Is it possible to isolate miRNA for TaqMan analysis? Thanks

11 November 2018 4,560 0 View

Homogenisation of tissue by Tissuelyser II?

Hi Has anybody protocol of isolation RNA (miRVana kit) using tissuelyser II? (Is the weight of tissue the critical value or I can add more lysis buffer like ratio 1:10, if I don´t know finally...

10 October 2018 8,209 2 View

Low absorption 260/230 in RNA-important for taqmanRNA?

Hi I have adsorption 260/230 in RNA samples betwen 2,1-1,3 (adsorption 260/280 is really good). How important is this information for my cDNA synstesis and taqman analyses. Can I use this samples?...

04 April 2018 5,960 1 View

Qubit microRNA Assay Kit for detecting in TotalRNA?

Hi everybody One question about Qubit microRNA Assay Kit. I isolated total RNA with using Mirvana microRNA isolation kit. Can i use Qubit mirna assay to detecting concentration of miRNA in total...

03 March 2018 5,916 2 View

Protocol for isolatio of Total RNA or small rna enrichment (Mirvana microRNA kit) in application in taqman mirna expression??

Hi I have a question about protocol of isolation RNA by Mirvana microRNA kit. I want to detect expression mirna in rats tissue of mammary gland tumor using of Taqman mirna. Is it correct use total...

03 March 2018 5,919 3 View