What test is used when the dependent variable has two levels?

Can MANOVA be used with: independent variables: 1. nominal with two levels will be dummy coded 0 and 1 2. ordinal with two levels, measured using a Likert scale Dependent Variables: 1....

03 March 2020 5,964 3 View

What can cause a dark current error on a spectrophotometer?

I have a UV-VIS spectrophotometer (VWR, UV-1600) which was out on a research cruise and when it came back to the lab and I tried starting it up, it passed all start up tests but fails the dark...

10 December 2019 7,469 3 View

Is it better to use PVDF or nylon membrane for cell culter filtering?

I need to order .2 um filters and don't know if I should order PDVF or nylon membranes. Any thoughts on which are better for neuronal stem cell medium?

19 July 2018 5,673 3 View

What are some studies that examine the effect of specific wording on task reminding, task initiation and prospective memory?

Why is there a difference between an item on a to-do list or calendar that says "Goto gym" versus one that says "Fit into tuxedo in time for wedding - work out at the gym now!" Which studies can I...

22 January 2016 8,186 6 View

Why are there double bands on western blots for mtor and p-mtor ser2448? Is there a biological explanation?

I am unsure of the cause. It is reproducible across multiple blots and cells lines with both the phospho-specific antibody and the total mTOR antibody. Thanks for the help!

24 August 2015 781 4 View

Interesting. I wonder if the learning impact of visuals in Maths is stronger in low level, medium level or high level students?

Visuals are commonly used to help the low level students understand maths but who's to say it can't benefit everyone.

01 January 1970 2,029 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View