20 July 2018 3 6K Report

I need to order .2 um filters and don't know if I should order PDVF or nylon membranes. Any thoughts on which are better for neuronal stem cell medium?

More Brandy Phillips's questions See All
Does anyone know the concentrations of the plate wash and dye release reagents in the Sircol 2.0 soluble collagen assay?

We used the wrong protocol and used all the plate wash and dye release reagents, but still have more of everything else. I wanted to see what to use so the rest of the kit doesn't go to waste.

02 April 2024 6,737 1 View

How can I measure the osmotic pressure/osmolarity of tissue culture media?

Hello! I want to measure the osmotic pressure/osmolarity of some tissue culture media. I have a freezing point osmometer which I use for liquid media, however I don't know if this will be...

09 January 2024 3,910 1 View

TEM analyis is required for Hydrogel morphology studies. How to prepare a film for TEM?

I am working with water insoluble Lignin-PVA hydrogel. TEM analysis is required for insight into its morphology. For TEM I am suppose to prepare a

29 September 2023 3,627 0 View

Resources for generating updated city-specific IDF curves with recent rain gauge data?

Hello fellow 'gators' (not sure if that's what we call ourselves...) I'm in need of some advice/links. I'm wanting to generate city-specific Intensity/Duration/Frequency data that accounts for...

14 August 2023 216 6 View

Do i need to gain permission to use online reviews in a content analysis for my research?

Hi everyone, I would like to perform content analysis on online reviews I collect from Tripadvisor, Google Reviews, and Facebook, but I am unsure if I need to gain permission from the companies...

01 May 2023 5,993 2 View

Polynomial regression or growth models?

I am trying to figure out the best/simplest way to explain why you should use a growth model for animal growth instead of taking a polynomial regression approach. I believe it is because...

14 March 2023 1,932 4 View

TAU-U online calculator for single case research- how do I compare baseline with a combined intervention and follow up?

I am using TAU-U online calculator (http://singlecaseresearch.org/calculators/tau-u) to complement my visual analysis for a multiple baseline single case design. My dataset has three phases...

18 February 2023 4,076 1 View

Tensile and DMA testing 3D printed specimens?

I am looking into two different materials, PEEK and ULTEM 9085. I want to test and compare the two (cyclic, tensile, etc) however ULTEM 9085 is only available to purchase in filament form, so I...

10 October 2022 567 2 View

In TAPPI T222 method for lignin estimation the wood biomass used is solubilized in 72% sulphuric acid or remain in suspended form?

I m trying to estimate the concentration of lignin in rice husk. I m using TAPPI T222 method for this purpose. On treating with 72% sulphuric acid, the husk powder is still visible. Is it correct...

17 August 2022 3,224 4 View

I m working on the synthesis of PEG hydrogel. Looking at methylene bisacrylamide as crosslinker. Can anyone suggest the ratio or concentration ofMBA?

Need to know the concentration of MBA in PEG hydrogel synthesis.

21 April 2022 5,724 1 View

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Issues with Malaria Coated Nitrocellulose Membrane Releasing Moisture?

Hello, I am currently working with Malaria Pf/Pan nitrocellulose membranes coated for malaria detection. After drying the coated membranes in an Incubator (overnight at 37°C), I've noticed that...

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How can we test a Nafion membrane ?

we have a nafion 117 membrane and we want to know if it has the properties claimed by the sellers. what are the tests available ?

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In kalman filter, it is logical to have the observation variance equale 0 ?

I am working on implementing a Kalman filter integrated with ARMA parameters, as described in the article "Predicting Time Series Using an Automatic New Algorithm of the Kalman Filter"...

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How to wash capsule filters (Membrane: polyethersulfone; pore size0.2u/0.45u) after filtration. can I wash by NaOH? if yes, what will be the molarity?

NA

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How to filter structural variants from WGS/WES data?

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