Greetings, I'm looking for literature related to a topic I want to write a manuscript on. I'm working with an glycine oxidase enzyme and noticed when I monitor the rate via spectrophotometry and a coupled-reaction, it has an initial period of ramping up before reaching the maximum rate for the reaction.

I've measured the length of this "lag time," both in terms of how long it takes to reach the maximum steady state rate, and also a half time. This lag time varies depending on both glycine and oxygen concentration. This enzyme likely has a ping-pong mechanism, where it binds glycine, oxidizes it, releases the glyoxylate and peroxide products, then the enzyme it re-oxidized by oxygen for another turn over. In anaerobic buffer, we can observe the first few steps of the reaction occur, glycine binding to the enzyme, but it stays there without oxygen. The enzyme itself has a yellow color due to a cysteine tryptophylquinone cofactor in the active site, and this changes to blue when bound with glycine.

Any way, I want to figure out if this pre-steady state lag time is due wholly to the fact that I'm using a coupled assay (I'm coupling the hydrogen peroxide production to consumption of NADH via glutamate dehydrogenase, and observing the loss of absorbance @340 nm), partially due to the coupled assay, or if I'm observing a trait wholly related to my enzyme. I want to find similar literature, but searching with phrases like "lag time/phase" bring up thousands of articles about bacterial growth characteristics, and basically I have been having trouble finding papers on this topic.

Thanks for any help.

Edit - I miswrote the coupled assay I'm using, we used to use Amplex Red, which reacted with the H2O2 product, but the glutamate dehydrogenase coupled reaction uses the ammonia product of the oxidized glycine