Kyle J. Mamounis

4 Questions 6 Answers 0 Followers

Questions related from Kyle J. Mamounis

Amino acid oxidase - amino acid binding modeling software, are SwissDock and AutoDock Vina the best?

I want to model amino acids in the active site of my amino acid oxidase flavoprotein and am going to try SwissDock and AutoDock Vina. Does anyone have experience with this type of modeling and...

06 December 2021 2,333 2 View

Is there literature on pre-steady state "lag" for oxidase enzymes?

Greetings, I'm looking for literature related to a topic I want to write a manuscript on. I'm working with an glycine oxidase enzyme and noticed when I monitor the rate via spectrophotometry and a...

01 April 2020 4,183 2 View

How do I set up an equation to calculate the amount of O2-saturated buffer to add to aerobic reagent mixtures to achieve particular O2 concentrations?

Out of convenience I want to keep my main buffer stock and reagents at aerobic conditions, seal a reaction mixture after combining the reagents, and add from a sealed O2-saturated buffer stock an...

27 October 2017 5,644 0 View

Suggestions on how to prevent precipitation of flavoprotein?

GoxB is a marine bacterial flavoprotein that catalyzes the covalent modification from precursor to mature form of a glycine oxidase (GoxA). Grown and expressed in E. coli Rosetta cells, it elutes...

20 September 2017 3,892 6 View