How to determine the optical rotation of a molecule that consist 1 chiral carbon?

How to know the wavelength that i should use to measure the optical rotation? What is the difference between optical rotation and optical ellipticity? thanks

02 March 2016 3,067 7 View

What is the best solvent to dissolve Glucomoringin (Glucosinolate) to run NMR?

My compound only dissolve in water (glucomoringin). what is the best solvent to dissolve it to run NMR

01 February 2016 7,424 2 View

Can someone help with Glucomoringin extraction and purification?

10 November 2015 7,584 4 View

Is it possible for MTT result to not be time dependent?

08 September 2015 8,826 4 View

Can anyone help with impurities in purified glucosinolates?

I had purify glucosinolates from seed of plant. But when i do NMR, the result showed impurities in 0-2.5 ppm. How can i solved this issue?

05 June 2015 1,737 5 View

What to do when compound did not dissolve in organic solvent?

03 April 2015 5,007 6 View

Any suggestions on thin layer chromatography and column chromatography?

i had done tlc for my crude extract as it has been suggested by sv to check the compound and solvent system. i had found only 1 spot for my crude and the best solvent system. my desired pure...

02 March 2015 8,964 17 View

Why do some people perceive the orchid as beautiful or attractive?

Why do people spending a lot of money for buying orchid? What are the characteristics of beautiful or characteristics of marketable orchids?

02 March 2014 5,512 1 View

What is the best procedure for pineapple culture n36?

We tried to culture pineapple n36 for some months, but still have fungus contamination. Any suggestion for sterilization techniques?

06 July 2013 8,339 5 View

EDC chemical handling?

I have to use the EDC chemical for my project. I have never used it before, I 'd read on the label for storage condition which stated that it can only be freshly use. Does it mean that I can't...

04 May 2013 3,934 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View