I tried to isolate stem cell from colon cancer cell line by magnetic beads method but the amount of the isolated cells was not enough also i want to know either after isolation can culture them again and passage and cryopreserve them again or not?
When your cell number is less, Yes you can grow them further up to P1/ P2 and further store them in LiqN2. Stem cell from cancer can be used till P8-P10. So, when ever you required take one vial and use up to P8. All the best