To isolate cells by their surface markers, which kits or product do I have to purchase for MACS method?

More Tarlan Jafarpour's questions See All

Is there anybody who isolated stem cells from colon cancer cell line?

I tried to isolate stem cell from colon cancer cell line by magnetic beads method but the amount of the isolated cells was not enough also i want to know either after isolation can culture them...

08 September 2014 2,626 3 View

Is there anybody who can help me to design a peptide?

I am going to make a peptide for cancerous cell line treatment.

01 February 2014 5,259 8 View

How many days before flow cytometry do I have to culture my cells in serum free media to detect stem cell markers?

I want to culture my cancerous cell line in serum free media to detect cancer stem cells by flow cytometry. How many days before doing flowcytometry would be suitable to culture them to be able to...

10 November 2013 5,695 5 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Wei Yang

which cell do you want to isolate? I'm using the kits from stem cell to isolate T cells right now. I think these kits are better than MACS, they don't need column, and can save more time. The purity and yield are also better than MACS.

Tarlan Jafarpour

i am going to isolate CD44 and CD133 cells.

Anirban Kar

I used MACS to isolate CD44 and CD133 labelled cancer stem cell population

Ruban Rex Peter Durairaj

I think this might be useful.

https://www.miltenyibiotec.com/en/products-and-services/macs-cell-separation/manual-cell-separation.aspx

Dear Airban Kar,would you please explain what materials and items and from which company you used for this method?