14 Questions 32 Answers 0 Followers
Questions related from Naveen Kumar
HI, We all know by literature by adding FCCP we can uncouple the mitochondrial membrane potential and increase the rate of respiration. When I tried/ titrated with a cell line, instead of increase...
01 January 2019 6,686 1 View
Hi, Could some one explain me " What is Corrected Total Cell Fluorescence (CTCF) ? " Does CTCF has any units? Thank you
09 September 2018 1,364 0 View
Hello friends, Is it okay to use regular tissue culture/cell culture 96 well plates for measuring absorbance at 450nm? Although the wave length is in visible range I am bit skeptical about that...
07 July 2018 6,489 2 View
Hi, I am using electroporation to transfect 661W cells with px459 plasmid in a crispr study. As I see I am loosing too many cells due to electroporation as i am trying to optimize the protocol. My...
04 April 2018 8,337 1 View
Hi, I am trying to ligate/insert the phosphorylated/annealed guide sequence into the Bbs1 digested CIP treated px459. I am getting the bacterial colonies on the AMP agar plate. but when I am...
03 March 2018 642 7 View
How the generation time of bacteria varies from doubling time? Could some one help me understand the concept. Thanks
02 February 2018 7,887 6 View
Hi, I have just started to learn/adapt CRISPR system for our research and I found few companies providing CRIPSR stable cell lines. Unfortunately, none of them explained to the level where a...
12 December 2017 2,574 2 View
During Transformation, before heat shock at 42 degrees for 60-90 sec we keep the plasmid and bacterial cell mixture on ice for 30 min. Will some one help me why we do that? Thanks in advance
03 March 2016 9,526 5 View
I am trying to dissociate primary neurospheres (mouse), which are cryopreserved in Liq.N2. I followed the standard procedure given by the supplier and the same procedure was followed in many...
02 February 2015 1,146 0 View
Dear Friends, I came across a question from my superior "what is single cell biology". I tried answering him saying that studying the biology of cells that are obtained from a single cell and he...
09 September 2014 6,222 5 View
We all know, during few cell culture experiments we use serum free media, we still add FBS (Fetal Bovine Serum) in some cases. We are also aware that FBS provides the growth supplements for the...
08 August 2013 3,441 30 View
We know the terms Ex vivo and In vitro are very close but they are not same.. non of the articles gives satisfactory information in differentiating these terms. They say Ex vivo is growing...
07 July 2013 1,946 46 View
Is it better to publish our research in a (paid) journal of a good publisher with good impact (by paying) or in a free journals with OA (low or no IF)? Which one is preferable (when both the...
07 July 2013 6,985 16 View
How do TBS (Tris) and PBS differ from each other?
06 June 2013 6,570 6 View
