Is there any way to isolate mycovirus from the environment, such as bacteriophage?

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Which basis set is recommended for enantiomers?

Which basis set is recommended for DFT of enantiomers?

29 March 2024 5,949 1 View

Do you know a PhD Scholarship in User Experience Design?

Hi All, I'm a master graduate in Industrial Design, with some publication in wearable design and design thinking, and lately, I've been a UI/UX Product designer for 3 years.... Now/ I'm Looking...

17 March 2024 4,665 1 View

How do I make a 2D plasma emissivity profile?

How do I make a 2D plasma emissivity profile? I have 1D plasma emissivity data and a plot, but I am struggling with 2D plotting in Matlab. Anyone could help me to solve this problem with an...

21 January 2024 3,292 0 View

How to find and plot the impurity cooling rate in tokamak plasma?

How to find and plot the impurity cooling rate (carbon, oxygen, and iron) in a tokamak plasma. In fact, I want to compute the plasma emissivity profile, which is a function of plasma density,...

15 November 2023 4,633 0 View

How to remove stop codon from a full mitogenome sequence?

Hi. This is my first time working with a full mitochondrial genome. This sequence was sequenced using Illumina and its consensus was produced using Geneious prime. My questions are: 1) After...

26 September 2023 7,654 3 View

What are the most effective immunoinformatics tools for predicting CTL epitopes in the S-protein of SARS-CoV-2?

biotechnology

27 August 2023 6,553 1 View

What are the most effective immunoinformatics tools for predicting CTL epitopes in the S-protein of SARS-CoV-2?

biotechnology

27 August 2023 2,146 1 View

Which process is correct for FRAP analysis of a plant extract?

Some papers refer to calculate FRAP value as FRAP value = = [(A1 − A0)/(Ac − A0)] × 2, where Ac is the absorbance of the positive control, A1 is the absorbance of the sample, and A0 is the...

13 August 2023 8,588 3 View

Which graphite powder to buy for Graphene Oxide and Graphene synthesis?

I could see options from companies such as Sigma Aldrich etc but I want to know which particle size etc to buy.. I shall be grateful to response from the community. Thanks!

07 August 2023 6,638 7 View

Comparative study of 5 rice farming areas?

Hi, I have a question regarding comparative analysis. I want to do a comparison of 5 research areas related to the average rice yield to see which of the five areas is the best in rice yield...

29 March 2023 8,103 1 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Can I detect the FLAG sequence of my AAV vector by qPCR?

I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...

14 July 2024 8,482 3 View

Intracerebroventricular (ICV) AAV inj.?

I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...

12 July 2024 781 0 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

Does anyone know how to improve calcium-imaging fluorescence with GRIN lenses?

Hey everyone, I've been working with GRIN lenses (Inscopix) for almost 1 year and still had no luck seeing strong fluorescence on them. When I look at them, I can see good blood flow and,...

08 July 2024 5,178 2 View

Why do I have two different bands from the same virus after PCR?

I get two different bands from BCMV after PCR

07 July 2024 2,112 1 View

Problems to find a region of a Virus gene?

We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...

01 July 2024 7,644 3 View

Does broad spectrum phage become narrow spectrum by time ?

In the host range analysis, I saw that a temperate phage that I isolated had an effect against a different strain, but later (2 years) it did not affect that strain. What could be the explanation...

25 June 2024 9,729 3 View

Why is my phage library obtained at round 2 of Biopanning viscous?

After incubation of the TG1 cells with the helper phage, I spun down the culture and precipitated the supernatant with PEG/NaCl. After this, I spun it down again and resupended the pellet in PBS....

21 June 2024 6,260 2 View